期刊论文

Proteomics Studies of Eomecon Chionantha Hance Roots

中文

湖南师范大学自然科学学报

2096-5281

2016

39

5

22-26,56

10.7612/j.issn.1000-2537.2016.05.004

基金项目：湖南省科技厅资助项目（2011NK3049） 湖南省教育厅资助项目（11C0832） 湖南师范大学博士基金资助项目（120506）

本校为其他机构

为用蛋白质组学方法初步分析血水草根蛋白质的表达,提取血水草根的蛋白质,经双向凝胶电泳分离、考马斯亮蓝染色后,切取分离较好的蛋白质点,经基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOFTOF）分析和数据库（IPI）检索,对蛋白质进行鉴定.成功鉴定了28个蛋白质点,其中具有明确功能的蛋白质17种,分别属于DNA复制酶、翻译调控蛋白、代谢酶、细胞结构蛋白;另外11种为未知功能蛋白.

To analyze the protein expression in Eomecon chionantha Hance roots with the proteomics approach, all proteins were extracted and separated by two-dimensional gel electrophoresis(2-DE), and stained with coomassie brilliant blue G250 to produce a high resolution and reproducible 2-DE image. Protein spots select-ed from this image were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry ( MALDI-TOF-TOF ) . The mass spectrometric data were used to identify the proteins through searches of the IPI protein sequence database. A tota...