Crossing Wolbachia‐infected males with uninfected females reduced the hatching rate by 28% compared with that when infected males were crossed with infected females. The number of eggs laid by Wolbachia‐free females was significantly lower than laid by infected females. Wolbachia had a dual effect on Velarifictorus aspersus reproduction, inducing incomplete cytoplasmic incompatibility and promoting the fertility of infected females. Abstract Wolbachia is a maternally inherited endosymbiont of the phylum α‐Proteobacteria that is found widely...
