期刊论文

Isolation, identification and characterization of a Cd resistant bacterium

中文

微生物学报

0001-6209

2019

59

1

11-24

10.13343/j.cnki.wsxb.20170632

国家重点研发计划(2016YFD0800705-02) 农业部与财政部科研专项[农办财函〔2016〕6号] 湖南省教育厅科研项目(15C1408) 湖南省重点学科建设项目(2006180).

本校为第一机构

【目的】从活性污泥中筛选耐镉(Cd)菌株,并研究其生长特性及对溶液中Cd~(2+)吸附的最佳条件,以期为重金属Cd污染水体的微生物修复提供菌株资源和应用技术参考。【方法】采用平板划线法,从活性污泥中分离、筛选、驯化出耐Cd菌株,通过16S rRNA基因序列分析及溶血试验、蛋白质毒素结晶试验进行初步鉴定,并采用单因素实验优化菌株的培养条件,通过正交实验确定菌粉吸附溶液中Cd~(2+)的最佳条件,同时利用SEM-EDS及FTIR分析探讨菌粉吸附Cd~(2+)的机理。【结果】经分离、驯化得到1株耐Cd细菌菌株,命名为H6,初步鉴定为蜡样芽孢杆菌(Bacillus cereus),最大Cd~(2+)耐受浓度为350 mg/L。菌株H6的最佳生长条件...

[Objective] We isolated and identified a cadmium resistant bacterium from activated sludge. Furthermore, we studied its growth characteristics and optimal biosorption conditions of Cd~(2+). This study was expected to provide strain resources and applied reference for microbial remediation of cadmium contaminated water. [Methods] The bacterium was isolated by streak plate and identified based on its 16S rRNA analysis, hemolysis testing and protein crystallization dying experiment. The growth conditions of the strain were determined by single fac...