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Enhanced Enzymatic Performance of β-Mannanase Immobilized on Calcium Alginate Beads for the Generation of Mannan Oligosaccharides

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成果类型:
期刊论文
作者:
Chen, Xinggang;Tian, Zhuang;Zhou, Hongbo;Zhou, Guoying;Cheng, Haina
通讯作者:
Zhou, GY;Cheng, HA
作者机构:
[Zhou, Guoying; Zhou, GY; Chen, Xinggang] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Art, Hunan Prov Key Lab Control Forest Dis & Pests, Key Lab Nonwood Forest Cultivar & Conservat,Minis, Changsha 410004, Peoples R China.
[Cheng, HA; Cheng, Haina; Zhou, Hongbo; Tian, Zhuang] Cent South Univ, Sch Minerals Proc & Bioengn, Key Lab Biomet, Minist Educ, Changsha 410083, Peoples R China.
通讯机构:
[Cheng, HA ; Zhou, GY ] C
Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Art, Hunan Prov Key Lab Control Forest Dis & Pests, Key Lab Nonwood Forest Cultivar & Conservat,Minis, Changsha 410004, Peoples R China.
Cent South Univ, Sch Minerals Proc & Bioengn, Key Lab Biomet, Minist Educ, Changsha 410083, Peoples R China.
语种:
英文
关键词:
alginate;mannanase;immobilization;kinetics;stability;mannan oligosaccharides
期刊:
Foods
ISSN:
2304-8158
年:
2023
卷:
12
期:
16
页码:
3089-
基金类别:
Conceptualization, H.Z. and H.C.; methodology, H.C.; software, X.C.; validation, X.C., Z.T., and H.Z.; formal analysis, X.C.; investigation, X.C.; resources, H.Z.; data curation, X.C.; writing—original draft preparation, X.C.; writing—review and editing, Z.T.; visualization, Z.T.; supervision, G.Z.; project administration, G.Z.; funding acquisition, G.Z. All authors have read and agreed to the published version of the manuscript. This research was funded by the National Natural Science Foundation of China, grant number 31971661, and the Natural Science Foundation of Hunan Province, China, grant number 2021JJ31145.
机构署名:
本校为第一且通讯机构
院系归属:
林学院
摘要:
Mannan oligosaccharides (MOSs) are excellent prebiotics that are usually obtained via the enzymatic hydrolysis of mannan. In order to reduce the cost of preparing MOSs, immobilized enzymes that demonstrate good performance, require simple preparation, and are safe, inexpensive, and reusable must be developed urgently. In this study, β-mannanase was immobilized on calcium alginate (CaAlg). Under the optimal conditions of 320 U enzyme addition, 1.6% sodium alginate, 2% CaCl2, and 1 h of immobilization time, the immobilization yield reached 68.3%...

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