In this study, cold-pressed peanut meal hydrolysate (also named as cold-pressed peanut meal peptone, CPMP)was prepared by sequential enzymatic hydrolysis using pepsin and alkali proteinase. The molecular mass distribution,proximate analysis and amino acid composition of CPMP were determined. Furthermore, its potential as an alternative tothe commercial peptone in microbial culture was evaluated. CPMP was mainly composed of < 5.0 kDa peptide fractions.CPMP was rich in Ala, Arg, Asp, and Cys, especially Glu. Escherichia coli grew better for 8 h in CPMP medium than inbeef peptone yeast medium. Ba...