In this paper, freeze-tolerant yeasts from common fermented foods were enriched by five freeze-thawing cycles and were isolated and purified by spread plate and streak plate methods. The growth characteristics, gas production characteristics and antioxidant activity against lipid oxidation in fish of the isolates were analyzed. A total of 25 yeast strains with freezing tolerance were obtained, of which, strains J3, J7, J8, J9, J12, J15, J18 and J25 exhibited good fermentation ability at low temperatures while J8, J12 and J18 inhibited lipid oxidation effectively. These 8 strains were identifie...