期刊论文

Effect of Cold Stress on the Metabolome of Wickerhamomyces anomalus with Cold Resistance

中文

食品科学

1002-6630

2019

40

12

190-195

10.7506/spkx1002-6630-20180621-416

本校为第一机构

通过甲氧胺、双(三甲基硅烷基)三氟乙酰胺(含1%三甲基氯硅烷)对6 株抗冷冻异常威克汉姆酵母(Wickerhamomyces anomalus)菌株的培养液进行衍生化,采用气相色谱-质谱法测定冷胁迫处理(4 ℃)对6 株耐冷冻W. anomalus酵母代谢物的影响。质量浓度超过10 mg/L的代谢物为49 种,其中糖、糖醇、糖酸、糖苷34 种,反映了W. anomalus复杂的糖代谢能力,且包括大量具有益生元活性的糖类;此外,还含有角鲨烯、百里香酚-β-D-葡萄糖苷、β-羟基-苯丙酸、异长叶烯,表明W. anomalus具有萜类、苯丙素物质代谢途径。采用代谢物组学数据处理方法识别W. anomalus在冷胁迫时的差异代谢物,甘油、角鲨烯、赤藓糖醇、半乳糖...

The effect of cold stress (4 ℃) on metabolites of six strains of the cold resistant yeast Wickerhamomyces anomalus was investigated in this paper. The fermentation broths of these yeast strains was derivatized with methoxamine and BSTFA-TMS, and then the products were determined by gas chromatography-mass spectrometry (GC-MS). A total of 49 metabolites with more than 10 mg/L concentrations were identi fied, including 34 saccharides, sugar alcohols and sugar acids, several of which had prebiotic activity. These results indicate that the W. anom...