期刊论文

中文

江西农业大学学报

1000-2286

2011

33

6

1228-1235

10.3969/j.issn.1000-2286.2011.06.035

国家林业公益性行业科研专项（201004003）；

本校为第一机构

对球孢白僵菌BbⅢ22固态发酵条件进行优化,选用单因素实验确定最佳基质配方及玉米粉含量、含水量、接种量、发酵温度、发酵时间的范围。采用Box-Behnken实验设计和响应面分析方法,对球孢白僵菌BbⅢ22固态发酵条件进行优化,得出最佳固态发酵条件为:玉米粉8.22 g/L,水分38%,接种量10.5%,发酵温度25.7℃,在此条件下球孢白僵菌BbⅢ22的产孢量达到了10.578×10~9个/g,为优化前的3倍。

The fermentation conditions for Beauveria bassiana BbⅢ22 were optimized.At first,the optimal matrix formula,corn flour concentration,water contentration,inoculation,fermentation temperature and fermentation time were determined by single factor experiments.By using Box-Behnken experimental design and response surface analytical methodology the optimum fermentation conditions were obtained： Corn flour 8.22 g/L,water content 38%,inoculation 10.5%,temperature 25.7 ℃.Under these conditions,the sporulation of BbⅢ22 was 10.578 ×109 in per garm,...