期刊论文

中文

江西农业大学学报

1000-2286

2010

32

3

541-546

10.3969/j.issn.1000-2286.2010.03.023

国家“十一五”科技支撑项目（2006BAD18B0204,2006BAD01A1706）； 国家自然科学基金（30371184）； 湖南省科学技术厅科技计划项目（06FJ4111）；

本校为第一机构

以构建的油茶近成熟种子cDNA文库和EST文库为基础,采用分子克隆技术,分离克隆1个14-3-3蛋白的全长cDNA序列,由1 156个核苷酸组成,5’非编码区57 bp,3’非编码区301 bp,开放阅读框长777 bp,编码259个氨基酸。该基因编码蛋白的分子质量为29.466 ku,等电点4.78,无信号肽序列,是非分泌蛋白,命名为Co-14-3-3a。推测的二级结构有9个α-螺旋,1个反平行的β-折叠,位于αC和αD之间。

A cDNA library and an EST library of Camellia oleifera were constructed.A 14-3-3 protein gene was cloned from Camellia oleifera.This gene is 1 156 bp in length,including 57 bp of 5＇untranslated region and 301 bp of 3＇untranslated region.It has an open reading flame of 777 bp,encoding a protein of 259 amino acid residues with protein molecular weight of 29.466 ku and isoelectric point of 4.78.No signal peptide sequence was found,which shows it＇s a non-secreted protein.This gene is named Co-14-3-3a.The speculated secondary structure of Co-14-3-3a consist...