期刊论文

Cloning and expression analysis of three ARF genes in Camellia oleifera

中文

植物生理与分子生物学学报

1671-3877

2021

57

5

1151-1162

10.13592/j.cnki.ppj.2020.0215

国家自然科学基金（31730016）；

本校为第一机构

ARF （auxin response factor）又称生长素响应因子,是生长素信号转导途径中调控生长素响应基因表达的转录因子,参与植物的生长发育。本文采用PCR技术从油茶中克隆得到3条ARF基因并将其分别命名为CoARF5、CoARF9和CoARF19,其全长编码序列（CDS）分别为2 835、2 076和3 234 bp,分别编码944、691和1 077个氨基酸。将油茶ARF蛋白与拟南芥ARF家族基因蛋白构建系统进化树,分析发现CoARF5与AtARF5、CoARF9与AtARF9、CoARF19与AtARF19亲缘关系最近。多序列比对结果显示, CoARF5、CoARF9和CoARF19蛋白均具有ARF家族特有的3个结构域:B3 DNA结合结构域、Auxinresp结构域和AUX/IAA结构域。实时荧光定量PCR结...

ARF, known as auxin response factor, is a transcription factor that regulates the expression of auxin response gene in auxin signal transduction pathway. ARF also participates in plant growth and development. Three ARF genes were cloned from Camellia oleifera by PCR and named CoARF5, CoARF9 and CoARF19, respectively. Their full-length coding sequences(CDSs) are 2 835, 2 076 and 3 234 bp, respectively, encoding 944, 691 and 1 077 amino acids. The phylogenetic tree was constructed by the ARF proteins of C. oleifera and the ARF family of Arabidops...