期刊论文

Li, M.

中文

植物生理与分子生物学学报

1671-3877

2016

52

8

1255-1262

10.13592/j.cnki.ppj.2016.0211

(112-0991):湖南省***计划 (0490016):中南林业科技大学博士科研基金 (QJ201512):中南林业科技大学青年基金 (Grant 112-0991):This work was supported by the One-Hundred-Talents Scheme of Hunan Province (Grant 0490016):the Doctoral Scientiifc Research Foun-dation of Central South University of Forestry and Technology (Grant QJ201512):Youth Foundation of Central South University of Forestry and Technology

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根据珙桐转录组测序结果,筛选到一个与原花青素合成相关的基因,通过克隆该基因片段并进行序列分析确定该基因编码一个珙桐MYB转录因子,将其命名为DiMYB1(GenBank登录号KR996175)。该基因开放阅读框全长为924 bp,编码产物包含307个氨基酸。对其编码产物的基本理化性质、亲水性和疏水性、保守结构域、亚细胞定位等方面进行了生物信息学分析和预测。对DiMYB1基因编码产物的氨基酸序列进行聚类分析表明,其与拟南芥MYB123转录因子的同源性最高。应用qRT-PCR分析该基因的表达模式发现,DiMYB1基因在珙桐紫色幼叶中的表达量最高,其次是雄蕊,在芽、茎、成熟叶片、苞片、果肉和种子中只有微量表达。另外,D...

According to the Davidia involucrata transcriptome sequencing data, a gene involved in proanthocyanin biosynthesis was selected. The target gene was identified to encode a MYB1 transcription factor in D. involucrata by cloning and sequence analysis and named as DiMYB1 (GenBank accesssion No. KR996175). The fragment of DiMYB1 includes a ORF (open reading frame) of 924 bp, which encodes a protein product of 307 amino acids. Bioinformatic analysis and prediction have been conducted to identify the physicochemical property, hydrophilia and hydropho...