期刊论文

Tan, X.-F.

中文

植物生理与分子生物学学报

1671-3877

2016

52

6

895-904

10.13592/j.cnki.ppj.2016.0092

This work was supported by National Forestry Public Welfare Industry Research Project of China 资助国家林业公益性行业科研专项重点项目

本校为第一且通讯机构

以油桐转录组数据中FATB1基因的cDNA片段为模板, 通过RACE技术克隆获得该基因的CDS, 命名为VfFATB1, 并进行了生物信息学分析, 同时通过Real-time PCR和亚细胞定位对该基因的表达模式和表达部位进行了研究分析。结果表明VfFATB1是一个由8个α-螺旋和10个β-折叠组成“hot-dog”折叠结构的二聚体, 与JcFATB1相似度高达92%; 该基因有FAT重要催化作用的半胱氨酸活性位点Cys 320和3个保守的组氨酸位点His 140/285/345; 该基因在油脂合成后期种仁中的表达量最高, 是幼根的30倍左右, 且在油脂合成过程中一直是上调状态; 该基因定位于叶绿体中。综上所述, VfFATB1是一个位于叶绿体中且含有多个催化功能...

In this research, we report the cloning and functional analysis of VfFATB1 in tung tree (Vernicia fordii) that shares high similarity with Jatropha curcasto FATB1 (fatty acyl-ACP thioesterase B1) gene, up to 92%. The encoded protein contains a typical domain of "hot dog" folds composed 8 α-helixes and 10 β-sheets. It also has one essential Cys320 residue and three conserved His140/285/345 of FAT catalytic sites. At the same time, it has highest expression in the seed kernel, about 30 times than radicle, and was significantly increased during ...