期刊论文

Zhang, L.

中文

植物生理与分子生物学学报

1671-3877

2016

52

7

1099-1109

10.13592/j.cnki.ppj.2016.0197

国家自然科学基金

本校为第一且通讯机构

甘油-3-磷酸酰基转移酶(glycerol-3-phosphate acyltransferase, GPAT)是三酰甘油生物合成Kennedy途径中的关键酶,在植物的油脂合成与抗逆等方面起着重要作用。本研究在转录组数据基础上,通过RACE技术,从我国重要木本食用油料树种油茶中克隆了2个GPAT基因CoATS1和CoGPAT6的全长cDNA序列,编码序列长度分别为1 353和1 488 bp,分别编码450和495个氨基酸。蛋白结构预测发现2个蛋白C-端都含有1个酰基转移酶结构域, CoGPAT6的N-端还具有1个类卤酸脱卤酶结构域。多序列比对和系统进化分析结果表明, CoATS1与茶树的CsGPAT聚为一类, CoGPAT6与芝麻的SiGPAT6聚为一类。实时荧光定量PCR分析表明, CoATS1和C...

As a functionally important enzyme in the Kennedy pathway involved in triacylgycerol (TAG) accumulation, glycerol-3-phosphate acyltransferase (GPAT) plays a key role in lipid biosynthesis and tolerance to abiotic stress in plants. Based on Camellia oleifera transcriptome data obtained before, we cloned two full length cDNA of GPAT, i.e. CoATS1 and CoGPAT6 via RACE method. The coding sequences of the two cDNA are 1 353 and 1 488 bp in length, encoding 450 and 495 amino acids, respectively. Prediction of protein structure showed that both CoATS1 ...