期刊论文

Cloning and expression analysis of CesA genes involved in seed abortion in dove tree (Davidia involucrata)

Li, M.

中文

植物生理与分子生物学学报

1671-3877

2016

52

10

1481-1490

10.13592/j.cnki.ppj.2016.0245

(0490016):中南林业科技大学博士科研基金 (QJ201512):中南林业科技大学青年基金 (Grant 0490016):This work was supported by the Doctoral Scientiifc Research Foundation of Central South University of Forestry and Technology (Grant QJ201512):Youth Foundation of Central South University of Forestry and Technology

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严重的种子败育是影响珙桐自然繁殖的重要原因之一,调控种子败育的分子机制及关键基因目前尚不清楚。根据本研究小组前期获得的珙桐种子转录组数据,筛选到22个转录本,分属14个纤维素合酶CesA编码基因,所有转录本在败育种子中均呈现上调表达趋势。分析珙桐种子中的纤维素含量发现,正常种子在发育过程中纤维素含量持续升高,而在败育种子中纤维素含量显著高于正常种子。挑选4个在败育种子中显著上调的CesA基因进行qPCR分析,结果表明它们表达模式相似,在败育种子中表达量均显著高于正常种子,且在正常种子发育后期上调表达。挑选其中表达差异最显著的c43681. graph_c0进行克隆获得882 bp片段。经序列...

Serious seed abortion is one of the important factors which restrict the sexual reproduction of dove tree (Davidia involucrata). However, the molecular mechanisms and key genes involved in seed abortion remain unclear. According to the transcriptome data of D. involucrata seed previously obtained by our group, 22 transcripts, belong to 14 CesA genes were selected due to all 22 transcripts showed up-regulated expression in the abortive seeds. We measured the content of cellulose in D. involucrata seeds and found that the cellulose content continuously increased in normal seeds during developmen...