期刊论文

Jin, X.-L.

中文

植物生理与分子生物学学报

1671-3877

2016

52

1

55-61

10.13592/j.cnki.ppj.2015.0535

(201404710):林业公益性行业科研专项经费项目 :湖南省“十二五”重点学科(风景园林学)(湘教发[2011]76号)。This work was supported by the Forestry Industry Research Special Funds for Public Welfare Projects(Grant 201404710) (Landscape Architecture) of Hunan Province(Grant 76[2011]):the Twelfth Five-Year Key subjects

本校为第一且通讯机构

以9个常绿杂交冬青品种的离体叶片为供试材料进行低温胁迫处理,用电导法配以Logistic方程计算出半致死温度(LT_(50)),并测定了超氧化物歧化酶(SOD)、丙二醛(MDA)、脯氨酸和可溶性蛋白各项指标,运用隶属函数法进一步分析其抗寒能力。结果表明,杂交冬青的相对电导率(Rec)随着温度的降低而上升, LT_(50)均在–34.91~–14.13 °C之间;低温胁迫下,随着温度的降低, MDA含量逐渐上升, SOD活性和可溶性蛋白含量呈先上升后下降的趋势,而脯氨酸含量呈先升后降或上升的变化趋势。综合LT_(50)和各指标隶属函数分析法得出抗寒性强弱顺序为: ‘Shamrock’>‘Forsteri’>‘Schworbel’s Compacta’>‘Anna’s C...

In order to evaluate the cold resistance of nine evergreen Ilex cultivars, the physiological mechanism of detached-leaf from nine evergreen Ilex cultivars under low temperature stress was studied. Their semi-lethal temperatures were calculated by relative electric conductivity (Rec) and logistic equation. Meanwhile, the contents of SOD, MDA, free proline and soluble protein were determined to provide their cold resistance. The results indicated that Rec increased as the droping of temperature, and LT50 was between -14.13℃ and -34.91℃. With th...