期刊论文

中文

植物生理与分子生物学学报

1671-3877

2007

33

1

61-70

10.3321/j.issn:1671-3877.2007.01.009

Acknowledgements： We are grateful to Professor Donglin Zhang of the University of Maine for revising this paper.国家林业局重点项目（No.2006-12）资助.

本校为第一机构

自交不亲和是显花植物的一种重要生殖生理现象，为探明中国梨的自交不亲和特性，对‘锦香’（Pyrus bretschneideri cv．Jinxiang）和‘鹅酥’（Pyrus bretschneideri cv．Esu）2个中国梨品种进行了基因组PCR特异扩增、S基因序列分析及田间杂交授粉试验。结果确定它们各含1个新S—RNA酶基因，分别命名为S37^-和S38^- RNase，GenBank序列号为DQ839238和DQ839239。生物信息学分析结果表明，S37^-和S38^- RNA酶的推导氨基酸序列与S1^-至S36^- RNA酶36个梨S基因具有相同的、高度保守的C1和C2区，但其高变区与S1^-至S36^- RNA酶差异较大，其中与S15的差异最小，只有3个氨基酸不同。在推导的氨基酸水平...

Self-incompatibility(SI) is an important in traspecific reproductive barrier in flowering plants.To identify the S-alleles of Chinese pear species(Pyrus pyrifolia,P.bretschneideri,P.ussuriensis and P.sinkiangenis etc.),S-RNase-specific PCR amplification,sequence analyses and field pollination tests were perbretschneideri as materials.Two new S-RNase genes were identified from the two cultivars.They were 1122 bp and 1058 bp in length,and designated as S37-RNase (GenBank accession no.DQ839238)and S38-RNase (GenBank accession no.DQ839239).By comparison of their deduced amino acid sequences with t...