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Selection and validation of suitable reference genes for RT-qPCR analysis in dove tree (Davidia involucrata Baill.)

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成果类型:
期刊论文
作者:
Ren, Rui;Huang, Fengzhen;Gao, Runxin;Dong, Xujie;Peng, Jiqing;...
通讯作者:
Li, Meng
作者机构:
[Huang, Fengzhen; Ren, Rui; Gao, Runxin; Peng, Jiqing; Dong, Xujie; Li, Meng] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha, Hunan, Peoples R China.
[Ren, Rui] Jiangxi Acad Agr Sci, Nanchang, Jiangxi, Peoples R China.
[Cao, Fuxiang] Hunan Agr Univ, Coll Hort & Landscape, Changsha, Hunan, Peoples R China.
通讯机构:
[Li, Meng] C
Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
Reference gene;Bract development;Seed abortion;Leaf color;Dove tree (Davidia involucrata Baill.)
期刊:
Trees-Structure and Function
ISSN:
0931-1890
年:
2019
卷:
33
期:
3
页码:
837-849
基金类别:
National Keypoint Research and Invention Program [2016YFD0600102]; Key Research and Development Project of Hunan Province [2017NK2252]; Doctoral Research Fund of Central South University of Forestry and Technology [0490016]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学与技术学院
摘要:
Key messageFifteen candidate reference genes were selected from the dove tree genome. Their performance as internal controls for RT-qPCR analysis was assessed in bracts at different developmental stages, normal and abortive seeds, and leaves of different colors. The applicability of the candidate reference genes was validated through detection of the expression levels of three target genes normalized by different reference genes.AbstractDove tree (Davidia) is a relic species and has some unique traits in its reproductive organs. The molecular mechanisms underlying the development of these orga...

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