期刊论文

中文

浙江林业科技

1001-3776

2019

39

2

81-86

10.3969/j.issn.1001-3776.2019.02.014

湖南省教育厅“十二五”重点学科资助项目(2011-76) 国家林业局重点学科(林人发〔2016〕21号) 中央财政林业科技推广项目(〔2015〕XT008).

本校为第一机构

为了探讨大叶榉树Zelkova schneideriana叶片黄酮类成分,以1年生大叶榉树叶片为材料,在超声波辅助下,通过单因素和Box-Behnken试验,探究并优化了大叶榉叶片黄酮类乙醇提取方法;采用常规显色法,对大叶榉树叶片中黄酮类物质种类进行初步鉴定。结果表明:(1)优化后的大叶榉树黄酮类提取方法为:乙醇浓度63%、液料比31:1mL·g^-1、提取时间1.7 h,大叶榉树黄酮类提取量为(31.067±0.11)mg·g^-1;(2)初步鉴定发现:大叶榉树叶片中含有黄酮、具有邻二酚羟基结构的黄酮类化合物、具有邻二苯酚羟基或兼有3-OH,4=O或5-OH,4=O的化合物;可能有黄酮醇、二氢黄酮、二氢黄酮醇等;不含查尔酮和橙酮。

On August 26, 2017, collections were implemented on1-3 leaves of 1-year Zelkova schneideriana in greenhouse for experiment on extraction of flavonoids. Optimization of ultrasonic extraction of flavonoids was designed by box-behnken with three factors at three levels. The result demonstrated that the best technology for extraction was as follows, ethanol concentration of 63%, liquid ratio of 31:1 mL·g^-1, extraction time of 1.7 hour, with the yield of flavonoids of(31.067±0.11)mL·g^-1. Preliminary identification of extracted flavonoids showed...