期刊论文

中文

微生物学通报

0253-2654

2017

44

11

2660-2668

10.13344/j.microbiol.china.170111

国家自然科学基金项目（No.31440070,31571874） 湖南省教育厅优秀青年基金项目（No.15B250）

本校为第一机构

【目的】分析枯草芽孢杆菌纳豆菌亚种Bna05菌株代谢产物中脂肽类物质的存在情况,并探讨它们在抗霉功能中所发挥的作用。【方法】利用特异性引物对Bna05菌株进行脂肽合成酶类基因片段扩增、测序和BLAST比对分析;通过平板抑菌圈区域取样法获得Bna05菌株的高抗霉活性代谢产物,对该产物进行反相高效液相色谱(RP-HPLC)分离;用琼脂微稀释法测定分离物的抗霉活性,并对活性分离物进行质谱鉴定。【结果】Bna05菌株含有sfp和srfAA基因,未检测到ituC、 ituD、fenD、fenACE、bymB、bymC基因;RP-HPLC分离得到3组抗霉活性物质F_2、F_3和F_4,F_2中未检测到脂肽类物质,从F_3和F_4中分别鉴定出两类Surfactin同系物...

[Objective] To isolate and identify lipopeptides in metabolites of Bacillus subtilis subsp.natto Bna05 and to assess antifungal activity of the identified lipopeptides. [Methods] Primers specific for lipopeptide synthetases were used to amplify DNA fragments from strain Bna05 by PCR. The amplified DNA fragments were sequenced and analyzed using BLAST against GenBank database. The antifungal metabolites were obtained from the inhibition zone formed between Bna05 and Aspergillus niger on modified PDA plate and separated by RP-HPLC. Antifungal activity of fractions separated by RP-HPLC was determ...