期刊论文

中文

中国植保导刊

1672-6820

2019

39

9

11-20

10.3969/j.issn.1672-6820.2019.09.002

湖南省重大科技专项(2016NK1001) 中国农业科学院科技创新工程(CAAS-ASTIP-2015-IBFC) 国家青年基金(31501653).

本校为第一机构

采用涂布平板法和对峙培养法,从油菜菌核病发生区健康油菜根际土中筛选到1株对油菜菌核病病菌具有较强生防效果的拮抗细菌HX-140.结合形态学特征、生理生化特性及16S rDNA序列分析,将菌株HX-140鉴定为多粘类芽孢杆菌(Paenibacillus polymyxa).菌株HX-140对供试8种病原真菌有显著的拮抗作用,抑菌率均大于60％,对温度、NaCl和pH的耐受范围较广;其浓度为1×108 cfu/mL的发酵液对油菜菌核病的防治效果最佳,在离体油菜叶片上对油菜菌核病的防治效果为65.51％,在盆栽试验中对油菜菌核病的防治效果为62.22％.

Strain HX-140,which had a good bio-control effect on Sclerotinia sclerotiorum,was isolated by spread plate method and dual culture method from the rhizosphere soil of healthy rape in rape sclerotinia rot occurring area.It was identified as Paenibacillus polymyxa by morphological,physiological and biochemical characteristics and 16 S rDNA analysis.Strain HX-140 showed a wide temperature,NaCl and pH tolerance,and had significant antagonistic effect on the 8 tested pathogens with more than 60%inhibition rate.Fermentation broth of strain HX-140(108 cfu/mL)showed the best controlling effect on S.sc...