期刊论文

中文

菌物学报

1672-6472

2023

42

11

2257-2268

10.13346/j.mycosystema.230089

This work was supported by the National Natural Science Foundation of China (32071765) and the Hunan Provincial Innovation Foundation for Postgraduate (QL20220175). *Corresponding author. E-mail: csuftlihe@163.com Received: 2023-04-14; Accepted: 2023-05-22

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果生刺盘孢是油茶炭疽病的主要致病菌。研究果生刺盘孢RNA结合蛋白基因CfNOP12的生物学功能，阐述果生刺盘孢致病的分子机制，为油茶炭疽病的防治提供理论基础。根据同源重组原理，在果生刺盘孢中敲除目标基因CfNOP12,PCR验证获得正确的突变体ΔCfnop12；进一步构建PYF11::CfNOP12回补质粒，导入突变体原生质体中，筛选成功互补菌株ΔCfnop12-C。对这些菌株进行生物学表型测定，发现突变体ΔCfnop12营养生长速率显著下降，分生孢子的产量及附着胞形成率显著降低；在含有细胞壁胁迫剂的PDA培养基上，突变体ΔCfnop12的抑制率相较于野生型和回补菌株显著升高，在低温条件下，突变体的生长速率表现...

Colletotrichum fructicola is a major causal pathogen of oil-tea tree. The purpose of this study is to investigate the biological function of the RNA-binding proteins CfNop12 of C. fructicola and provide a theoretical basis for the prevention and control of oil-tea tree anthracnose. Based on the principle of homologous recombination, the CfNOP12 gene knockout vector fragment was constructed and transformed into the protoplast of C. fructicola to get the mutant strain of ∆Cfnop12. The mutant strain was verified by PCR amplification. The PCR-ampl...