期刊论文

Identification of lipid components in Ganoderma lingzhi byultra high performance liquid chromatography-electrospraytandem quadrupole mass spectrometry

中文

菌物学报

1672-6472

2023

42

05

1175-1184

国家自然科学基金(31900027,31772374,32071673) 中国博士后科学基金(2020M682601) 湖南省科技创新计划资助(2020RC2059,2021RC4063) 湖南省自然科学基金(2020JJ5972) 湖南省教育厅科学研究项目(22A0188,18B167)。

本校为第一机构

脂质是灵芝重要活性成分之一，但目前对灵芝胞内脂质成分的构成研究甚少。本研究采用UPLC-ESI-MS/MS技术，对灵芝发酵菌体的胞内脂质构成进行分析。结果显示，灵芝细胞中共鉴定到296种脂质，其中甘油酯112种、磷脂148种、鞘脂34种和甾醇2种；甘油酯和磷脂分别占总脂质的44.70%和38.06%，鞘脂和甾醇分别占总脂质的17.08%和0.16%。分析甘油酯中主要成分为甘油三酯，占甘油酯总含量的67.36%；磷脂中主要成分为磷脂酰乙醇胺，占磷脂总含量的62.64%；鞘脂中主要成分为神经酰胺，占鞘脂总量的60.33%；此外，本研究检测出27种游离脂肪酸，其中20种为不饱和脂肪酸，相对含量为65.59%;7种为饱和脂肪酸，相...

Lipids are one of the most important active components of Ganoderma lingzhi.However,there are few studies on the composition of intracellular lipids in G.lingzhi.Here,the intracellular lipid composition was analyzed by UPLC-ESI-MS/MS technique from G.lingzhi mycelium ce...MORE Lipids are one of the most important active components of Ganoderma lingzhi.However,there are few studies on the composition of intracellular lipids in G.lingzhi.Here,the intracellular lipid composition was analyzed by UPLC-ESI-MS/MS technique from G.lingzhi mycelium cell.The results showed that 296 kinds of lipids were ...