期刊论文

The putative vacuolar sorting protein CfVps17 is involved in regulating development and pathogenicity of Colletotrichum fructicola

中文

菌物学报

1672-6472

2021

40

8

2102-2113

10.13346/j.mycosystema.210051

国家自然科学基金（32071765）～～；

本校为第一机构

果生刺盘孢是油茶炭疽病的主要流行致病菌。本文研究果生刺盘孢中液泡分选蛋白CfVps17的生物学功能,为阐明该蛋白调控致病分子机制提供依据。采用over-lap方法构建CfVPS17基因敲除载体片段,使用PEG介导法将敲除载体片段转化至果生刺盘孢原生质体中,通过验证筛选获得突变体菌株ΔCfvps17。构建目的基因CfVPS17回补载体pYF11::CfVPS17,经转化获得ΔCfvps17-C回补菌株。测定野生型、突变体ΔCfvps17及回补菌株ΔCfvps17-C的生物学表型,结果表明,与野生型和回补菌株相比,突变体ΔCfvps17菌丝生长速率、产孢量和附着胞形成率显著降低,突变体菌丝中糖原积累减少,对氯化钠和刚果红胁迫耐受性增强;致病力...

Colletotrichum fructicola is a major pathogen causing anthracnose on Camellia oleifera. This experiment aims at revealing the biological functions of the putative vacuolar sorting protein CfVps17, providing a basis for illuminating the pathogenic molecular mechanism of C. fructicola regulated by this protein. The over-lap method was used to construct the CfVPS17 gene-deleted fragment which was transformed into the protoplasts of wild-type strain CFLH16 by PEG-mediated method for obtaining mutant strain ΔCfvps17 through validation screening. A ...