期刊论文

Signal transduction and gene expression analysis of triterpenoid acid synthesis induced by 9,10- cyclomethylheptadecanoic acid in Ganoderma lingzhi

中文

菌物学报

1672-6472

2021

40

9

2317-2329

10.13346/j.mycosystema.210079

基金项目：国家自然科学基金（31772374）；国家重点研发计划政府间国际科技合作重点专项（2017YFE0108100）；湖 南省高校创新平台开放基金（17K106）；湖南省林业科技创新项目（XLK201831，XLK201944）；湖南省科技创新团队项 目（2021） Supported by the National Natural Science Fundation of China (31772374), National Key R&D Programs of Intergovernmental International Science and Technology Cooperation (2017YFE0108100), Hunan University Innovation Platform Open Fund (17K106), the Forestry Science and Technology Innovation Project in Hunan Province (XLK201831, XLK201944), and Hunan Provincial Science and Technology Innovation Team (2021). Corresponding authors. E-mail: zhangshaobing@csuft.edu.cn, gaoliuedu@csuft.edu.cn Received: 2021-02-26, accepted: 2021-06-17

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为探究9,10-环甲基十七烷酸(9,10-CMA)诱导灵芝三萜酸合成的相关信号机制,分析了NO信号的介导作用。结果表明,在NO信号分子介导下,9,10-CMA可有效地刺激灵芝菌丝体中三萜合成关键酶细胞色素CYP450和苯丙氨酸解氨酶(PAL)的活化以及三萜酸的合成。NO可作为灵芝菌丝体中CYP450产生和PAL活化的上游分子发挥作用。在9,10-CMA诱导下,通过荧光定量PCR分析了6个关键酶基因在灵芝三萜酸合成过程的动态表达。结果表明,在9,10-CMA诱导下与灵芝三萜酸合成相关的角鲨烯合成酶基因sqs、细胞色素P450单加氧酶CYP5150L8基因和3-羟基-3-甲基戊二酰辅酶A还原酶基因hmgr的上调最显著,提示这3个酶在三萜诱导过程中具...

In order to study the signal mechanism of 9,10-cyclomethylheptadecanoic acid (9,10-CMA) - induced triterpenoid acid (TA) synthesis in Ganoderma lingzhi, the mediating role of NO signal was analyzed. The results showed that under the induction of 9,10-CMA, NO could effectively trigger the activation of phenylalanine ammonia lyase (PAL) and cytochrome CYP450, and the synthesis of triterpenoid acids in G. lingzhi mycelia. Nitric oxide plays an inducer as a upstream molecule of CYP450 and PAL activating in G. lingzhi cell, but it is not the only si...