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组蛋白去乙酰化酶CfSnt2调控果生刺盘孢生长发育和致病力

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成果类型:
期刊论文
作者:
陈振宏;李河;陈嘉怡;陈晓迪;罗晶;...
作者机构:
[陈振宏; 李河; 陈嘉怡; 陈晓迪; 罗晶; 张盛培] Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
语种:
中文
关键词:
油茶炭疽病;果生刺盘孢;组蛋白去乙酰化酶CfSnt2;基因敲除;生长;致病力
关键词(英文):
anthracnose on Camellia oleifera;Colletotrichum fructicola;histone deacetylases CfSnt2;gene knockout;growth;pathogenicity
期刊:
菌物学报
ISSN:
1672-6472
年:
2022
卷:
41
期:
6
页码:
926-938
基金类别:
Supported by the National Natural Science Foundation of China (32001317), and the Research Foundation of Education Bureau of Hunan Province (18B172). *Corresponding author. E-mail: csuftzsp@163.com Received: 2021-09-03, accepted: 2021-10-28
机构署名:
本校为第一机构
院系归属:
林学院
摘要:
炭疽病是油茶Camellia oleifera的重要病害,该病害的优势致病菌是刺盘孢属Colletotrichum的果生刺盘孢Colletotrichum fructicola,在全国的油茶产区普遍发生。我们前期发现组蛋白乙酰转移酶CfGcn5调控油茶果生刺盘孢的生长发育和致病过程,但组蛋白去乙酰化酶在该病菌中的生物学功能尚不清楚。本研究以组蛋白去乙酰化酶CfSnt2为研究对象,利用反向遗传学的方法,通过对野生型、CfSNT2基因敲除突变体及互补菌株的生物学表型进行比较分析,发现CfSNT2基因敲除突变体的菌丝生长速率明显减缓、分生孢子的产量显著减少、附着胞形成率降低、对细胞壁胁迫剂的响应异常,同时对油茶致病力显著减弱。以...
摘要(英文):
Anthracnose is one of the most important diseases occurring on Camellia oleifera. The dominant pathogen causing the disease is Colletotrichum fructicola which is common in C. oleifera producing areas all over the country. Previous study recognized that the histone acetyltransferase CfGcn5 regulated growth, development and pathogenicity of C. fructicola, but the functions of histone deacetylases remain unknown. In this study, the reverse genetics method was adopted to comparatively analyze the biological phenotypes of wide-type, CfSNT2 gene knoc...

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