期刊论文

Effects of some exogenous substances on polysaccharide and β‐glucan syntheses of Ganoderma lingzhi in submerged fermentation

中文

菌物学报

1672-6472

2020

39

1

164-173

10.13346/j.mycosystema.190322

基金项目：国家重点研发计划政府间国际科技合作重点专项（2017YFE0108100）；湖南省国际科技创新合作基地项目 （2018WK4008）；湖南省自然科学基金（2017JJ2406） Supported by National Key R & D Programs of Intergovernmental International Science and Technology Cooperation (2017YFE0108100), Foundation of Hunan International Cooperation Base of Scientific and Technological Innovation (2018WK4008), and the Natural Science Foundation of Hunan Province (2017JJ2406). Corresponding authors. E-mail: wxlcsedu@163.com, gaoliuedu@163.com Received: 2019-08-22, accepted: 2019-09-18

本校为第一机构

研究了14种外源物质(化合物)对灵芝细胞生长和发酵合成多糖和β-葡聚糖的影响.结果 表明,连翘水提物(3g/L)对灵芝细胞生长具有显著促进作用;薏苡仁酯(3g/L)对灵芝胞内多糖和β-葡聚糖的合成均具有促进作用;而桔梗水提物、硝酸铈铵、硝酸镨、茉莉酸甲酯和硝普钠对灵芝细胞生长和产物合成均具有抑制作用.进一步通过Box-Behnken试验设计和响应面法分析,建立了添加薏苡仁酯发酵产β-葡聚糖的二次多项式模型,经分析得到产β-葡聚糖的最优条件为:薏苡仁酯添加量10.5g/L、接种量16％、添加时间第88小时、发酵初始pH 7.00.在此条件下获得β-葡聚糖的产量可达(40.67±8.43)mg/L,与未添加薏苡仁酯的对照组...

The effects of 14 additives or compounds on the growth and synthesis of polysaccharides and beta-glucan of fermentative Ganoderma lingzhi were studied. The results showed that the aqueous extract of Forsythia suspensa fruits (3g/L) had significant promoting effect on the growth of G. lingzhi, and coixenolide (3g/L) had stimulatory effect on the synthesis of intracellular polysaccharides and beta-glucan. However, the water extract of Platycodon grandiflorum roots, cerium ammonium nitrate, praseodymium nitrate, methyl jasmonate and sodium nitropr...