期刊论文

Enhancement of gibberellin acid production through pH regulation in batch fermentation of Gibberella fujikuroi

中文

菌物学报

1672-6472

2019

38

7

1185-1190

10.13346/j.mycosystema.190108

Supported by the Research Foundation of Education Bureau of Hunan Province, China (15C1418). Corresponding author. E-mail: wanderwish@126.com Received: 2019-04-04, accepted: 2019-04-26

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为高效率发酵生产GA_3,对藤仓赤霉菌发酵过程pH 进行优化调控研究。采用5L 全自动发酵罐,在pH 3.0–5.0条件下,对藤仓赤霉菌菌丝生长及GA_3产量的影响进行了考察,实验数据表明:在pH 4.0条件下,菌比生长速率可获最大值,为0.395/h;而pH 3.0条件下,GA_3比生成速率最大,达到4.43mg/(g×h)。基于不同pH 条件下,对菌比生长速率、得率、GA_3比生成速率的影响,提出GA_3分批发酵过程中的pH调控策略,即:0–20h,pH 自然;20–50h,pH 4.0;50–80h,pH 3.0–3.5;80h 后控制pH 为3.5–4.0。在此控制模式下,经过196h 发酵GA_3的终产量达到2224mg/L,GA_3产率44.5mg/g,GA_3生产强度0.242mg/(L×h),分别比不控制pH 条...

The effects of medium pH on the mycelium growth and GA3 production of Gibberella fujikuroi fermented in 5L automatic bioreactor were investigated. The experiment results showed that under the condition of pH 4.0, the maximum growth rate of strain ZNL-13 was 0.395/h, while under pH 3.0 the GA3 production rate reached a maximum of 4.43mg/(g∙h). It was proposed that in batch fermentation the medium pH value should be regulated as follows on the basis of fermentation process: 0–20h, natural pH; 20–50h, pH 4.0; 50–80h, pH 3.0–3.5; over 80h, pH ...