期刊论文

中文

菌物学报

1672-6472

2017

36

5

611-617

10.13346/j.mycosystema.160172

(15C1418):湖南省教育厅一般项目 (15C1418):Supported by the Research Foundation of Education Bureau of Hunan Province of China

本校为第一机构

为了解溶氧对赤霉素发酵过程影响以及相应工艺优化,采用不同溶氧条件下藤仓赤霉菌Gibberella fujikuroi分批发酵生产赤霉素的过程进行菌丝浓度、残糖浓度和GA_3产物浓度检测,并微分运算得出比生长速率与比产物合成速率随发酵时间变化,分析了溶氧对比生长速率与比产物合成速率以及得率的影响,进而提出Gibberella fujikuroi发酵高产的溶氧控制策略:在发酵初始阶段(0–50h)控制溶氧30%左右,以维持较高的菌体生长速率;发酵中后期(50–184h),溶氧控制在15%,以获取菌丝持续较高的GA_3合成速率能力。采用这一优化溶氧控制策略,发酵过程中最大菌丝浓度19.24g/L、最终赤霉素浓度2180mg/L和平均比产物合成...

In order to understand the effects of dissolved oxygen (DO) on gibberellin acid (GA_3) fermentation and obtain the optimization DO control strategy, the batch fermentation experiments of GA_3 production by Gibberella fujikuroi were studied under the different DO conditions. The mycelium concentration, residual sugar concentration and GA_3 concentration in the fermentation broth were detected opportunely. Then, through the calculation of the differential derivation, the mycelium specific growth rate and the specific product synthesis rate were o...