期刊论文

Tissue Culture and Highly Efficient Rooting of Kalmia latifolia 'Olympic Fire'

中文

云南农业大学学报(自然科学)

1004-390X

2017

32

1

89-94

10.16211/j.issn.1004-390X(n).2017.01.013

(K1205016-21):长沙市科技计划项目 :湖南省“十二五”重点学科(风景园林学)(湘教发[2011]76号) (林人发[2016] 21号):国家林业局重点学科资助项目

本校为第一机构

生根困难一直是影响山月桂应用的主要问题,本研究以山月桂‘奥运圣火’带芽茎段为外植体,研究基本培养基、植物生长调节剂,以及珍珠岩对其腋芽萌发、组培苗的增殖、伸长和生根的影响。结果表明:最适合茎段腋芽萌发的培养基为WPM + 1.0 mg/L 2-ip,萌发率可达72.5%;组培苗增殖的最佳培养基为 WPM + 1.0 mg/L 2-ip + 0.05 mg/L NAA,增殖系数可达5.42,平均苗高达3.42 cm;最适合的生根培养基为:1/2 WPM + 1.0 mg/L IBA + 0.1 mg/L NAA +珍珠岩,生根率80.03%。将已经生根的组培苗炼苗后移栽到泥炭土和珍珠岩(1:1,体积比)的混合基质中,成活率在60%左右。

Difficulty in root generation hinders the application of Kalmia latifolia. By in vitro culture of nodal segments of K. latifolia ＇ Olympic Fire＇ , the effects of basal medium, plant growth regulators and perlite on rooting for rapid propagation were investigated. The results indicated that WPM basal medium supplemented with 2-ip 1.0 mg/L significantly improved bud break and growth directly from nodal segment explants, and the sprouting rate was about 72.5%. The optimal medium for shoot multi- plieation was WPM ＋ 1.0 mg/L 2-ip ＋ 0. 05 mg/L N...