期刊论文

中文

生物加工过程

1672-3678

2011

9

3

52-56

10.3969/j.issn.1672-3678.2011.03.011

“十一五”国家科技支撑计划资助项目（2006BAD07A07-08）；

本校为第一机构

以大肠杆菌和金黄色葡萄球菌为模式菌,对AgNO,的抗茵效果进行研究,并对其抗茵机制作初步探讨。 AgNO_3对大肠杆菌的抑制生长曲线表明:2.891 mg/L的AgNO_3能够完全抑制10~6个/mL的大肠杆菌细胞生长,AgNO_3使大肠杆菌和金黄色葡萄球菌的延滞期加长,并且浓度越高,延滞期越长。另外,AgNO_3对大肠杆菌和金黄色葡萄球菌脱氢酶的活性有明显影响,随着AgNO_3浓度的提高,脱氢酶的活性逐渐降低。AgNO_3溶液作用于细菌后,细菌表面疏水性均有不同程度地下降,且浓度越大对其影响也越明显,大肠杆菌的下降程度要大于金黄色葡萄球菌。

Using Escherichia coli and Staphylococcus aureus as model strains,the antibacterial effect and the mechanism of silver nitrate were investigated.Experimental results indicated that the silver nitrate of 2.891 mg/mL could inhibit completely the growth of 106cfu/mL cells in liquid LB medium,and that silver nitrate prolonged the lag phase of E.coli and S.aureus,the higher the concentration,the longer the lag phase.In addition,silver nitrate had a marked effect on the dehydrogenase activity of E.coli and S.aureus,the dehydrogenase activity decreased as the concentration increased.The surface hydro...