期刊论文

中文

光谱实验室

1004-8138

2009

26

3

530-534

10.3969/j.issn.1004-8138.2009.03.019

湖南省教育厅基金资助项目（07C811）；

本校为第一机构

采用加热回流法提取川桂叶中的黄酮类化合物,建立了川桂叶中总黄酮含量测定的光谱分析方法.结果表明:以芦丁为标准品,用NaNO_2-Al(NO_3)_3-NaOH显色法可测定川桂叶中总黄酮的含量,该方法的精密度和准确度均较高,相对标准偏差为0.63%,方法的回收率为97.18%-102.20%.通过正交试验优化,得到提取川桂叶中黄酮类化合物的最佳工艺条件是:用体积分数为70%的乙醇作溶剂,在固液比1:20(g/mL)、提取温度80℃的条件下提取1.5h,连续提取2次.铡得川桂叶中总黄酮含量为22.53mg·g~(-1).

Flavonoids were extracted from Cinnamomum wilsonii Gamble leaves by Heating refluxing, and determined by spectrophotomertry. The NaNO3-Al （NO3）2-NaOH method is practicable to determine flavonoids content from C. wilsonii Gamble leaves with rutin as the standard. This method is higher precision and accuracy with RSD 0. 63% and recoveries 97. 18%-102.20%. The optimum extraction conditions were obtained by orthogonal experiments as follows：extracting solvent 70% （V/V） ethanol, material ratio 1 ： 20 （g/mL）, extraction temperature 80℃, extr...