期刊论文

Quantification and fast-screening of malachite green in aquaculture water using paper spray ionization mass spectrometry

中文

卫生研究

1000-8020

2020

49

01

107-111

10.19813/j.cnki.weishengyanjiu.2020.01.019

湖南省卫生计生委科研基金（No.C2015-064）；

本校为第一机构

目的采用纸喷雾质谱( paper spray ionization mass spectrometry,PSI-MS)技术,建立养殖水中孔雀石绿( malachite green,MG)的快速定量筛查方法。方法以氯仿为萃取剂,低共熔溶剂( deep eutectic solvents,DESs)为助溶剂,快速提取MG,氘代孔雀石绿( malachite green D_5,MG-D_5)同位素内标稀释定量,PSI-MS检测,C_(18)色谱柱( 4.6 mm×250 mm,5 μm) 。结果方法工作曲线范围0.1 ~ 10 μg /mL(相关系数>0.999), 检出限0.03 μg /mL,定量限0.1 μg /mL,日内精密度3.5%,日间精密度6.2%,加标回收率98.9%~106.9%( n = 7), 单个样品PSI-MS检测耗时1 min,检测结果与液相色谱-串联质谱法( liquid chromatog...

OBJECTIVE A rapid quantitative technique had been developed for the screening of malachite green ( MG) in aquaculture water using paper spray ionization mass spectrometry ( PSI-MS). METHODS To rapid extract MG,chloroform and deep eutectic solvents ( DESs ) were used as extraction agent and co-solvent respectively. malachite green D_5 ( MG-D_5 ) was used as an internal standard to quantitative determination MG,C_(18) chromatographic column ( 4.6 mm×250 mm,5 μm). RESULTS The method showed a good linearity ( R~2 = 0.999 ) in the concentration ra...