期刊论文

Isolation and expression of floral organ development regulating gene LiFUL1 in Lagerstroemia indica L

中文

浙江农业学报

1004-1524

2020

32

7

1206-1214

10.3969/j.issn.1004-1524.2020.07.09

kq1801026:长沙市科技计划 2019YFD1001000:国家重点研发计划

本校为第一机构

紫薇湘韵具有只开花不结实的特性,表现为花药不开裂、花粉败育、胚珠发育异常。紫薇红叶为普通紫薇,可正常开花结实。以紫薇湘韵为试验材料,采用RT-PCR方法,从花芽中分离得到1个FRUITFULL(FUL)同源基因,命名为LiFUL1(GenBank登录号为MN894547)。序列分析结果表明,其cDNA开放阅读框长度为753 bp,编码251个氨基酸,分子质量为28.453 13 ku。序列比对和保守结构域分析结果表明,LiFUL1基因编码蛋白具有典型的MADS-MEF2和K-box结构域,C末端含有一个保守性高的基序euFUL MOTIF,因此,LiFUL1属于MADS家族的FUL/AP1亚家族。进化分析表明,紫薇的LiFUL1基因编码的蛋白氨基酸序列与木本植物蓝桉的FUL/AP1氨基...

Flowers of crape myrtle Xiangyun are not strong enough to seeding, the manifestations of Xiangyun are indehiscent anther, pollen abortion and abnormal ovule. Redleaf is common crape myrtle with normal flowering and bearing. Crape myrtle Xiangyun was selected as the experimental material, RT-PCR was employed to isolate a homologous gene of FRUITFULL (FUL) from Xiangyun, named as LiFUL1(GenBank accession No. MN894547). Sequence analysis indicated that length of the open reading frame of LiFUL1 cDNA was 753 bp, which encoded a protein with 251 amino acids, its molecular weight was 28.453 13 ku. A...