期刊论文

Study on Rapid Propagation via Tissue Culture of Curculigo

中文

特产研究

1001-4721

2017

39

4

6-12

10.16720/j.cnki.tcyj.2017.04.002

国家林业公益性行业科技专项项目（201304807）；

本校为第一机构

以仙茅幼叶为材料,采用不同的激素诱导出愈伤组织,进而产生不定芽,不定芽继代产生小植株,小植株生根后移栽入土成活,从而建立了仙茅快繁技术.结果表明,MS+2,4-二氯苯乙酸(2,4-D) 0.1 mg/L+KT 1.5mg/L+多菌灵3％+柠檬酸8mg/L诱导愈伤组织效果最好,可达96％.继代培养基为MS+NAA0.3mg/L+6-BA2mg/L+2,4-D0.3mg/L,继代效果最好、增殖率最高、愈伤组织的质量最好,较易诱导出不定芽.在培养基中添加0.3mg/L的NNA有利于生根,移栽至营养土中的成活率可达到90％以上.

Callus was induced by different hormone concentrations in young leaves of Curculigo orchiodes.The suitable medium was se- lected to induce adventitious buds, and then the rooting experiment was carried out and transplanted to soil.The results showed that MS＋2,4-D0.1 mg/L＋KT1.Smg/L＋carbendazim 3%＋citric acid 8mg/L was the best in MS medium.Up to 96%.The subculture medium was MS＋NAA0.3mg/L＋6-BA2mg/L＋2,4-D0.3mg/L, the best subculture was the MS, the highest callus quality was the best, More easily induced adventitious buds.Adding 0.3mg/L NN...