Tree peony is a traditional woody flower originating from China and possesses high ornamental value. The yellow cultivar is even more precious, known as the highest grade. However, the molecular mechanism underlying tree peony yellow flower formation is still unclear. Our present work identified two transcription factors (TFs), PsMYB4 and PsEGL3, which were highly expressed in yellow tree peony cultivar. Phylogenetic analysis indicates that PsMYB4 belonged to the R2R3-MYB repressor, while PsEGL3 was clustered into subgroup IIIf of bHLH family. Overexpression of PsMYB4 and PsEGL3 respectively i...