期刊论文

Simultaneous determination of tetracyclines,fluoroquinolones,and sulfonamides in chicken manure using solid-phase extraction and high performance liquid chromatography

Chen, C.

中文

色谱

1000-8713

2019

37

5

518-524

10.3724/SP.J.1123.2018.11006

2017YFD0800700:国家重点研发计划 2016YFD0800202:国家重点研发计划

本校为第一且通讯机构

建立了一种高效、低成本的固相萃取-高效液相色谱(SPE-HPLC)同时测定鸡粪中6种常见抗生素(2种四环素类、2种喹诺酮类和2种磺胺类)的分析方法。样品经EDTA-McIlvaine缓冲液和有机混合提取液(甲醇-乙腈-丙酮,2∶2∶1,v/v/v)提取,过HLB固相萃取柱净化,甲醇-二氯甲烷(7∶3,v/v)洗脱,高效液相色谱-二极管阵列检测器测定,检测波长λ=270 nm,柱温32 ℃,流动相为乙腈-0.7%(v/v)磷酸水溶液。该方法在0.5~100 mg/L质量浓度范围内的标准曲线相关系数r~2在0.9999~1之间,样品加标回收率在70.0%~116.3%之间,相对标准偏差为1.2%~16.6%。方法检出限为1.3~6.7 μg/kg,定量限为3.5~9.2 μg/kg。应用该方法对辽宁省...

A highly efficient and economic method for the simultaneous determination of six common antibiotics (two tetracyclines, two quinolones, and two sulfonamides) in chicken manure was developed by using solid-phase extraction and high performance liquid chromatography (SPE-HPLC). The samples were extracted by using a mixture of the EDTA-McIlvaine buffer and a mixed organic extractant (methanol-acetonitrile-acetone, 2:2:1, v/v/v), cleaned by a hydrophile-lipophile balance (HLB) cartridge, eluted by methanol-dichloromethane (7:3, v/v), and separated ...