会议论文

The expression, cloning and analysis of chlorophyll-binding protein cab genes in Ginkgo biloba

中文

2011

63-72

全国第十九次银杏学术研讨会

全国第十九次银杏学术研讨会论文集

2011-09-21

浙江长兴

中国林学会

本校为第一机构

为了从分子水平研究银杏光合作用的机理,采用EST文库方法,揭示了银杏叶绿素结合蛋白基因cab表达规律.同时,通过对所测EST序列拼接组装,从银杏中分离了叶绿素结合蛋白cab家族的一个基因,命名为GbCab1.GbCab1基因的cDNA序列全长为904 bp,含有一个完整的编码序列CDS(Coding sequence),CDS长度为813 bp(72～884bp),编码271个氨基酸.序列分析结果表明,GbCab1基因是光系统Ⅱ的一个捕光叶绿素结合蛋白基因,与禾本科植物玉米(Zea mays)捕光叶绿素a/b结合蛋白基因cab-m7有较高的同源性,相似性达到85％.系统进化树分析表明,GbCab1基因与银杏(Ginkgo biloba)PSⅡcab基因、樟子松(Pinus sylvestris cab)基因...

　　The expression of Ginkgo biloba chlorophyll-binding protein gene cab was revealed,using EST library method, in order to study on the molecular level mechanism of Ginkgo biloba photosynthesis.By Assembling the ESTs from clones similar to PSII chlorophyll a/b binding protein gene through CAP3 programm, a new gene called GbCab1 was got as a candidate gene of PSII chlorophyll a/b binding protein (cab).In comparison with cab genes from other plants using BLASTX, GbCab1 may be identified as a new cab gene, which is a full-length cDNA sequence wit...