期刊论文

中文

中国油脂

1003-7969

2016

41

6

10-14

10.3969/j.issn.1003-7969.2016.06.003

本校为第一机构

以老化黄秋葵的籽为原料,采用二次通用旋转组合试验优化水酶法制备黄秋葵籽油工艺,并通过Rancimat法分析产品的氧化稳定性。结果表明:碱性内切蛋白酶(最适酶解条件:55℃、pH 8.5)适用于水酶法制备黄秋葵籽油;该工艺的最佳条件为料液比1∶5.5、酶解时间4.2 h、酶添加量3%(以底物质量计),此条件下的黄秋葵籽油得率为(14.25±0.06)%;随着温度的升高,黄秋葵籽油的诱导期逐渐缩短,在常温(25℃)时,水酶法制备的黄秋葵籽油货架期230 d要短于溶剂浸出法制备的281 d,长于冷榨法制备的164 d。

With mature okra seeds as raw material,the aqueous enzymatic extraction process of okra seed oil was optimized by quadratic general rotary combination design,and the oxidation stability of okra seed oil was analyzed by Rancimat method.The results showed that Alcalase(optimal enzymolysis conditions:55 ℃,p H 8.5) was suitable for aqueous enzymatic extraction of okra seed oil,and the optimal process conditions were obtained as follows: ratio of material to liquid 1 ∶ 5.5,enzymolysis time 4.2 h,dosage of Alcalase 3%(based on substrate mass).Under...