This study aimed to analyze spatio-temporal changes in habitat quality in Chang-Zhu-Tan Urban Agglomeration during the 2000-2020 period and explore its topographic gradient effects. Using land use data from this period, the InVEST model was employed to assess the spatio-temporal variations in habitat quality. The bivariate spatial autocorrelation model was used to analyze the spatial correlation characteristics between habitat quality and various topographical factors. Additionally, terrain factor analysis was utilized to study the terrain gradient effects on habitat quality in the study area....