Tidal flow constructed wetlands (TFCWs) enhance the process of microbial denitrification, while, the influence of substrate pore structure on denitrification are not clear. Herein, we prepare macroporous zeolite ball (MZ) by pore construction technique, and equipped as substrate in TFCWs, to explore the influence of MZ on the structure and function of microbial community. Results show ed that MZ has porous morphology both on the surface and inside. The removal rate of NH4+-N was 12% higher than that of the control. Surface characteristics of MZ provided more attachment sites for microorganisms...