期刊论文

Optimization of immobilization conditions of Bacillus cereus using sodium alginate combined with corn straw biochar

中文

环境化学

0254-6108

2019

38

1

106-113

10.7524/j.issn.0254-6108.2018013002

国家重点研发计划(2016YFD0800705-02) 农业部与财政部科研专项(农办财函[2016]6号) 湖南省教育厅科研项目(15C1408) 湖南省重点学科建设项目(2006180)资助.

本校为第一机构

为探讨耐Cd菌株实际应用的可能性,用海藻酸钠( SA)联合自制的玉米秸秆炭包埋固定耐Cd菌株蜡样芽孢杆菌H6,通过单因素实验与正交实验,优化了包埋固定的最佳配比,并以1 mol·L~(-1)的HNO_3溶液作为解吸剂,考察了固定化小球的重复利用效果.结果表明,在实验设定的条件下,固定化小球的最佳制备条件为: SA浓度4%,包炭量2%,包菌量25%,交联剂CaCl_2浓度2.5%.SA、玉米秸秆炭和菌三者组合制备的小球对 Cd~(2+)的吸附能力强于SA或SA单独包埋炭或菌,最高Cd~(2+)吸附量可达5.80 mg·g~(-1) .解吸实验结果表明,固定化小球在解吸4次后仍具有较强的吸附能力.因此,固定化小球能有效循环利用且吸附能力较稳定.

To explore the possibility of application of cadmium-resistant strains,the optimal preparation conditions were investigated by the single factor and orthogonal experimental design through the immobilized biosorption of cadmium-resistant strain H6( Bacillus cereus) and corn straw biochar by sodium alginate.The effect of recycling of the immobilized beads was determined by the desorption experiments with 1 mol·L~(-1) aqueous nitric acid as the desorption agent. Bosed on the results,the optimal preparation conditions of the immobilized beads were...