On the basis of single factor test,the extraction process of polyphenols from Acorn shuck was studied by orthogonal test through the polyphenols content determined by Prussian Blue specIrophotometry.From the variance analysis,the order influenced extraction of polyphenols was extraction temperature >extraction time>volume fraction of solvent >ratio of solid to liquid.The results showed that the optimal extraction conditions were 60 % acetone aquatic solution as extractant,1∶30 of the ratio of solid to liquid,...