期刊论文

中文

经济林研究

1003-8981

2017

35

3

35-43

10.14067/j.cnki.1003-8981.2017.03.005

(201204403):国家林业公益性行业科研专项重大项目

本校为第一机构

为给油桐ALDH基因的结构与功能研究提供理论依据,以葡萄桐的近成熟种子为材料,根据油桐转录组测序结果设计引物,采用RT-PCR技术克隆了油桐ALDH22A1基因和ALDH2C4基因的全长cDNA序列。ALDH22A1的cDNA序列全长1 782 bp,编码593个氨基酸,该基因编码蛋白质的相对分子质量为65.60 kDa,理论等电点为6.62,蛋白二级结构以α螺旋为主,具有1个明显的跨膜结构,是稳定的非分泌蛋白。ALDH2C4的cDNA序列全长1 506 bp,编码501个氨基酸,该基因编码蛋白质的相对分子质量为54.82 kDa,理论等电点为6.58,蛋白二级结构以α螺旋为主,是不具有跨膜结构的膜外蛋白。BLASTp分析结果表明,这2个基因所编码序列与麻疯树、蓖麻...

In order to provide some theoretical basis for the studies on structure and function ofALDH gene in Vernicia fordii, using nearly ripe seeds in ＇Putaotong＇ as materials, primers were designed according to the results of the transcriptome sequencing analysis of V.fordii, and full-length eDNA sequences of ALDH22A1 and ALDH2C4 genes were cloned by RT-PCR techniques. The eDNA sequence length ofALDH22A1 gene was 1 782 bp, encoding 593 amino acids. The relative molecular mass of ALDH22A1 protein was 65.60 kDa, and the theoretical isoelectric point ...