期刊论文

中文

经济林研究

1003-8981

2016

34

4

47-51

10.14067/j.cnki.1003-8981.2016.04.008

国家林业公益性行业科研专项（201304406） 国家林业局软科学研究项目（2013.R09） 湖南省自然科学创新研究群体基金（湘基金委字（2013）7号）和城市森林生态湖南省重点实验室资助项目.

本校为第一机构

为给杜仲SSR分子标记的选择提供参考依据,利用MISA软件对杜仲转录组序列进行了微卫星查找,共获得重复单元长度为1~6碱基的29 067个微卫星序列。分析这些微卫星序列后发现,在杜仲转录组中,长度为二核苷酸的微卫星重复单元最为丰富,占43.7%;在单碱基重复和二碱基重复这两种类型中,最主要的优势重复单元分别是A/T和AG/CT;在三碱基、四碱基、五碱基重复类型中,（AAN）n、（ANAT）n、（AANAN）n分别为其对应的优势重复单元。杜仲转录组中,微卫星的频率随着微卫星总长度的增加而降低。

In order to provide some references for the choice of SSR molecular markers of Eucommia ulmoides, microsatellites in the transcriptome sequences of E. ulmoides were found by MISA software. A total of 29 067 microsatellites were identified with repeating units at the length of 1-6 bases. The microsatellite sequences were analyzed. The results showed that dinucleotide microsatellite repeating units were the most abundant in E. ulmoides transcriptomes, which accounted for 43.7%. Among the two repeating types of mononucleotide and dinucleotide, the...