期刊论文

中文

经济林研究

1003-8981

2016

34

1

158-163

10.14067/j.cnki.1003-8981.2016.01.026

国家“十二五”农村领域科技计划课题研究任务合约（2013BAD14D0502）；

本校为第一机构

为给阳丰甜柿的无性繁殖提供参考,采用不同灭菌方法处理完全甜柿阳丰的不同外植体,对外植体类型、树龄、取材时间及灭菌处理等对灭菌效果的影响进行研究。结果表明:以1年生阳丰甜柿当年生枝条的带皮腋芽作为外植体进行组织培养效果最好,生长速度快,而休眠芽在剥去5片鳞片后组培效果也较好,但后期生长速度较慢。当年生枝条的带皮腋芽的最佳灭菌方法是:75%酒精处理30 s,0.1%升汞处理15 min;剥掉5片鳞片后的休眠芽茎尖最佳灭菌方法:75%酒精处理1 min,5%次氯酸钠处理15 min,0.1%升汞处理20 min。带皮腋芽最佳取材时间是6月,接种后外植体的污染率和死亡率显著低于它取材时间,而芽的萌发率显著高于其它...

In order to provide technical support for asexual reproduction of Diospyros kaki cv. Youhou, using different sterilization methods to deal with different explants from D. kaki cv. Youhou, effects of explants type, tree age, sampling time and sterilization method on sterilization results were researched. The results showed that taking axillary buds with skin of annual shoot as explants, sterilization effect was best, and the explants fast grew, followed by dormant buds stripped five scales, but the latter slowly grew. The best process of sterili...