In order to obtain aseptic living explants in Zelkova schneideriana, seeds in Z. schneideriana were seeded in sand and medium after sterilization. Aseptic shoots can be gain from seeds with low vigor and no natural germination ability by the method of callus induction and differentiation. Aseptic seedlings from seeds with high vigor by direct germination. Aseptic sowing of seeds in Z. schneideriana is an effective...