In order to improve preparation quantity and separation degree, the preparation condition of polyphenol in Camellia pericarp was optimized by semi-preparation HPLC. The results showed that after extracting polyphenol with 50% of alcohol, the optimization condition of isolating and purifying polyphenol by semi-preparation HPLC was as follows: gradient elution was carried on by mobile phase A (volume ratio of water to acetic acid was 100 : 1) and B (acetonitrile) at 2.5 mL/min flow rate, the injection volume was 0.4 mL. The content of singl...