In order to realize rapid propagation in Chirita langshanica,taking tender leaves as experimental materials,tissue culture techniques in Chirita langshanica were studied under different culture conditions.The results indicated that MS supplemented with BA 1.0 mg·L-1 and NAA 0.5 mg·L-1 was the optimal medium for inducing callus,MS supplemented with BA 0.5 mg·L-1 and NAA 0.3 mg·L-1 was the best medium for cluster buds differentiation,and MS supplemented with NAA 0.3 mg·L-1 and quartz sand was the optimal medium for inducing root. Hardening s...