期刊论文

Determination of 9 kinds of fatty acids in vegetable oil by HPLC-ELSD

中文

食品与机械

1003-5788

2021

37

07

51-56+93

10.13652/j.issn.1003-5788.2021.07.008

湖南省科技重大专项（编号：2018NK1030）；

本校为第一机构

目的:探索一种无需衍生就能准确得到植物油中脂肪酸的绝对含量的脂肪酸测定方法。方法:通过高效液相色谱与蒸发光散射检测器联用方法对植物油中主要脂肪酸含量进行更准确的分析测定,采用CNW? C18-WP色谱柱（4.6 mm×250 mm, 5μm）;流动相A为0.1%甲酸水溶液,流动相B为0.1%甲酸甲醇溶液,采用梯度洗脱,流速为1.0 mL/min;柱温箱温度设为25℃,进样量为30μL;蒸发光检测器漂移管温度为75℃;无油空气泵提供载气,流速为2.0 L/min;增益设为4。结果:在该条件下,十四酸、软脂酸、亚麻酸、亚油酸、油酸、硬脂酸、花生酸、芥酸和山嵛酸都有较好的线性关系,且稳定性高（RSD均在3%之内）、精密度好（RSD均在3%...

Objective:Due to the blackness of sensitivity,selectivity and fatty acid quantification of traditional methods,a new method for determining fatty acids in vegetable oils without derivation was developed.Methods:The content and types of fatty acids in vegetable oil were analyzed more accurately by high-performance liquid chromatography combined with evaporative light scattering detector,CNW C18-WP column(4.6 mm×250 mm,5μm)was used.Mobile phase A was 0.1%formic acid aqueous solution,and mobile phase B was 0.1%formic acid methanol solution,using...