期刊论文

Optimization of two luteolin derivatives preparation from artichoke by HPLC

中文

食品与机械

1003-5788

2019

35

3

176-181

10.13652/j.issn.1003-5788.2019.03.032

湖南省自然科学基金(编号:2016JJ5016) 中南林业科技大学大学生研究性学习与创新性实验计划项目(编号:2018075).

本校为第一机构

研究了一种通过制备液相色谱从朝鲜蓟中分离得到2种木犀草素糖苷的方法.朝鲜蓟与70％乙醇浸提液通过AB-8型大孔树脂吸附,分别用20％,50％,80％乙醇洗脱,得到50％ 乙醇洗脱部位A27～29(4 g);A27～29用RP-C18层析柱(270 mm×80 mm,40～60μm)吸附,分别用30％,48％,100％甲醇洗脱,得到C16～19目标组分0.32 g,其中含目标组分H-01(10.4％)和H-02(5.1％);以目标组分的分离度和峰形为优化条件,研究了5种不同流动相组合及2种流速对目标峰分离效果的影响,基于最优条件制备,得到了纯度分别为97.8％ 和96.5％ 的H-01和H-02;通过质谱、1 H-NMR和13 C-NMR表征,确定H-01和H-02分别为木犀草苷-7-芸香糖苷和木犀...

This study describes a method for isolating two luteolin glycosides from artichokes(Cynara scolymus L.)by preparative liquid chromatography.The partion A27~29(4 g)of 50% ethanol eluent was achieved by two steps,70%ethanol extract of artichoke adsorbed by AB-8 macroporous resin and eluted with20% ethanol,50% ethanol and 80% ethanol successively.0.32 g of target part C16~19 with target components H-01(10.4%)and H-02(5.1%)was achieved by two steps,A27~29 adsorbed by RP-C18 stationary phase column(270 mm×80 mm,40~60μm)and eluted with 30%,48% and ...